FIGURE 7.

(A) Efficient detection of ManLAM via capture assays. ManLAM in buffer was captured in 96-well microtiter plates coated with CS-35, the FIND Abs, or P30B9 and detected with biotinylated A194-01 IgG using a sensitive ECL detection assay based on an AP-conjugated streptavidin reagent. (B) Demonstration of the ability to detect LAM in unprocessed urine from a panel of TB⁺/HIV-1⁻ patients, using a sensitive ECL assay based on an HRP-conjugated streptavidin reagent. (C) Signals obtained for titered concentrations of ManLAM diluted in buffer tested in this assay for comparison. A linear correlation was seen in this assay for the range of Ag concentrations tested and relative luminescence units (RLUs), with a lower limit <0.05 ng/ml.