Fig. 3.

Effect of BSA and Tween 20 addition on SSCF of EDA-pretreated CS. a 6% glucan loading; b 9% glucan loading. SSCF conditions were temperature 34 °C, pH = 4.8, inoculum size 2.5 g dry cell/L, 12-h pre-hydrolysis, and 150 rpm shaker speed. Ctec2 and Htec2 loadings were both 10 mg protein/g glucan. The concentration of BSA and Tween 20 was 0.1 g/L. Error bars represent SD, n = 3, *P < 0.05, **P < 0.01