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. 2018 Apr 16;9:312. doi: 10.3389/fphys.2018.00312

Figure 1.

Figure 1

Gene and protein expression of the androgen receptor (AR) in human breast cancer cell lines and effects of antiandrogens on the viability of MDA-MB-453 cells. (A) Real-time PCR assay for AR in seven human breast cancer cell lines (n = 3 for each). (B) Expression of AR proteins (approximately 110 kDa) in MDA-MB-453, YMB-1, MCF-7, and MDA-MB-231 cells. Protein lysates of the examined cells were probed by immunoblotting with anti-AR (upper panel) and anti-ACTB (lower panel) antibodies on the same filter. (C) Expression of AR transcripts in MDA-MB-453 cells cultivated for 5 days with normal FBS- and charcoal-stripped FBS-supplemented (10%) medium (n = 4 for each). (D,E) Effects of treatments with the antiandrogens, bicalutamide (BCT, 1 μM) and enzalutamide (EZT, 1 μM) for 72 h on the viability of MDA-MB-453 cells pre-cultivated for 5 days with normal FCS- (D) and charcoal-stripped FCS- (E) supplemented medium. The viability of vehicle-treated cells is arbitrarily expressed as 1.0, and data are shown as “relative cell viability” (n = 5 for each). Expression levels were expressed as a ratio to ACTB (A,C). Results were expressed as means ± SEM. **p <0.01 vs. the normal FBS group or vehicle control.