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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Am J Transplant. 2017 May 30;17(11):2810–2819. doi: 10.1111/ajt.14328

Figure 2.

Figure 2

Eight hours of donor cold ischemia induces C3-dependent, post-transplant inflammation. A. WT B/c allografts were subjected to 8h CIS and transplanted into WT (filled bars) or c3−/− B6 (open bars) recipients. Graft RNA was isolated 48 h later and gene expression analyzed by qPCR. Results are expressed as fold change relative to WT BALB/c hearts subjected to 30′ CI and transplanted into WT B6 recipients (set at 1, dashed line). *p<0.05 vs. 30′ CI, **p<0.05. n= 6–8 animals/condition performed in triplicate. B–D. Serum cytokine levels (multiplex Luminex analysis) 48 h after transplantation of BALB/c hearts exposed to 30′ or 8 h CI and transplanted into WT B6 or c3−/− B6 recipients. *p<0.05, n= 4–6 animals/condition. ND=none detected