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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Am J Transplant. 2017 May 30;17(11):2810–2819. doi: 10.1111/ajt.14328

Figure 3.

Figure 3

Cold ischemia-induced graft infiltration of primed CD8+ T cells is recipient C3-dependent. A. Experimental design schematic. B. Representative flow plot of graft infiltrating cells (gated on lymphocytes) 48 h post-transplant demonstrating detection of adoptively transferred CD45.2+CD8+ T cells. C–D. Quantification of CD45.2+ 2C CD8+ T cells within WT BALB/c grafts (C) or in recipient spleens (D) 48 h post-transplant into WT or c3−/− B6 recipients as indicated. (E) Quantification of host CD45.1+CD8+ T cells within graft infiltrating lymphocytes of the same animals. *p<0.05 as compared to BALB/c→ WT B6 8h CI. n= 6 animals/condition. NS: non-significant.