Figure 2.

Use of 405 nm rather than 488 nm laser increases signal intensity over the relevant range of angles for SSC detection. (a) Displays signal intensities calculated by computer modelling (Y axes) of light scatter around small particles at the indicated particle diameters. Light scatter was modelled at a collection angle of 31.5°–148.5° which is the range over which the side scatter signal is detected (X axes) for 405 nm (black dotted lines) and 488 nm (solid grey lines). These calculations were done for 100, 200 and 500 nm sized modelled spherical small particles (left to right) with refractive indexes (RIs) of 1.34, 1.37, 1.4 and 1.46 (top to bottom). (b) Depicts the overall fold changes in area under the curve (AUC) for 405 and 488 nm wavelengths for particles ranging in size from 100, 200 and 500 nm (Y axis) with RIs of 1.37 or 1.46 (X axis).