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Workflow employed for the production, fractionation, and characterization of Trypanosoma cruzi EVs and vesicle-free fraction from Y and YuYu strains. The details of each step are explained in the Materials and Methods section. DMEM, Dulbecco’s Modified Eagle’s Medium; NTA, nanoparticle tracking analysis; SEM, scanning electron microscopy; SEC, size-exclusion chromatography.
