Figure 7.
Effect of EVs on macrophages. J774 macrophages (105) were pre-incubated for 1 h with EVs from Y and YuYu strains containing each 1 or 10 µg of protein per mL, equivalent to 106 and 107 or just RPMI medium used as control. Cells were then incubated for 2 h with trypomastigotes (10 parasites/cell), washed and the incubation proceeded for more 24 h. The cells were fixed and stained with DAPI and the number of intracellular parasites determined in cells containing from 0 to 5 parasites/cells (a), 6 to 10 parasites/cells (b), 11 to 25 parasites/cells (c), and 26 to 50 parasites/cells (d). The values are means of duplicate experiments.