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. Author manuscript; available in PMC: 2019 Jan 15.
Published in final edited form as: J Immunol. 2017 Dec 6;200(2):768–774. doi: 10.4049/jimmunol.1701048

Figure 1. Human CD14+ monocytes produce IFN in response to Pf gDNA and infected RBCs.

Figure 1

(A) CD14+ monocytes were isolated from PBMCs from human healthy donors by MACS and transfected with Pf gDNA (10 μg/ml) using Lipofectamine 2000 or incubated with uninfected RBCs (uRBCs) or Pf infected RBCs (iRBCs) at a 20 to 1 RBC per monocyte ratio. RNA was prepared after 6 h incubation or transfection and IFNβ and β-actin levels were measured by qRT-PCR. (B) Human MDMs from healthy donors were transfected with natural Hz (50 μM), AT5 and AT5 3X ODNs (3 μM) or Pf gDNA (0.5 ml total at 1 μg/ml). IFNβ and β-actin levels measured as in (A). ISD, p(dA:dT) and Sendai virus (SeV) were used as positive controls. All samples were compared to cells incubated with medium alone or between specific groups when indicated and analyzed by Mann-Whitney U test. Data are presented as mean ± SD and are representative of 3 independent experiments. * indicates a p value of < 0.05, ** indicates a p value of < 0.001.