Fig. 4.
Activating Y416 phosphorylation of Src kinase in Bmpr2+/− but not Bmpr2ΔEx2/+ mouse PECs. ciPECs were isolated from WT control, Bmpr2+/−, and Bmpr2ΔEx2/+ mice. Western blot using Clone 18 anti-BMPR2 antibodies, pY416, and total Src kinase, and β-actin in WT, Bmpr2+/−, and Bmpr2ΔEx2/+ ciPEC lysates, as indicated. Reduced WT Bmpr2 expression seen in both Bmpr2+/− and Bmpr2ΔEx2/+ ciPECs compared to WT cells. Bmpr2ΔEx2/+ ciPECs also express a prominent Bmpr2ΔEx2 mutant product. pY416 Src kinase is only detected in Bmpr2+/− ciPECs. BMPR2 western blot was modified from Fig. 1 in Frump et al. with permission from the publishers.27 Corresponding pY416 and total Src kinase western blot studies have not previously been published.