Fig. 4.

A concentration-response heat map for comparatively evaluating acute locomotor behavioral responses to flavonoids and flavonoid-like compounds. All 24 chemicals in Table 2 were tested and a standard concentration range of 1, 5, 10, 25, and 50 µM was evaluated. Locomotor activity was binned into 30 min blocks and normalized to the respective time-bin control group (0.1% DMSO). Shaded cells indicate the normalized mean percent activity of animals relative to controls only when significantly different from control groups and exceeding a 1.5 fold change cutoff threshold. Significance determined using two-way repeated measures ANOVA with Tukey’s post-hoc, p ≤ 0.05, N = 16 animals per treatment group. A Euclidian distance metric with complete linkage was used for hierarchical clustering analysis (HCA). Summary statistics for all 24 flavonoids tested are provided in Supplementary Materials.