Labeled structure
|
Marker
|
Expression and localization
|
Class
|
Stock number
|
Comment
|
Ref.
|
Muscle
|
Mef2-GAL4 |
all myoblasts and all muscles at all stages |
GAL4 line |
BL 27390 |
|
5
|
1151-GAL4 |
adult muscle precursors and early myotubes until ≈24 h APF |
GAL4 line, enhancer trap |
- |
|
6
|
Act79B-GAL4 |
jump muscle upon differentiation |
GAL4 line |
- |
|
7
|
Act88F-GAL4 |
indirect flight muscles starting ≈14 h APF |
GAL4 line |
- |
|
7
|
Act88F-Cameleon 3.1 |
indirect flight muscles starting ≈14 h APF |
Act88F enhancer/ promoter driving Cameleon 3.1 |
- |
Ca2+ indicator |
8
|
Act88F-GFP |
indirect flight muscles starting ≈14 h APF |
GFP-fusion (fly TransgeneOme line) |
fTRG78 and fTRG10028 |
|
4
|
Him-nls-GFP |
adult muscle precursors, nuclear, until ≈24 h APF in indirect flight muscles |
enhancer/promoter with nls-GFP reporter |
- |
1.5 kb enhancer fragment |
9
|
Mhc-Tau-GFP |
microtubules in DLM templates and in differentiating muscles |
enhancer/promoter with Tau-GFP reporter |
BL 53739 |
|
10
|
βTub60D-GFP |
microtubules in myotubes (e.g. in indirect flight muscles from ≈14 h AFP, strongly decreasing after ≈48 h APF) |
GFP-fusion (fly TransgeneOme line) |
fTRG958 |
|
4
|
Mhc-GFP (weeP26) |
sarcomeres (thick filament) in all body muscles (e.g. in indirect flight muscles starting from ≈30 h APF) |
GFP-trap |
- |
use heterozygous, labels an isoform subset |
11
|
Sls-GFP |
sarcomeres (Z-disc) in all body muscles (e.g. in indirect flight muscles starting from ≈30 h APF) |
GFP-trap (FlyTrap line) |
- |
G53, use heterozygous |
2
|
Zasp66-GFP |
Z-disc in all body muscles |
GFP-trap (FlyTrap line) |
BL 6824 |
ZCL0663 |
2
12
|
Zasp52-GFP |
Z-disc in all body muscles |
GFP-trap (FlyTrap line) |
BL 6838 |
G00189 |
2
12
|
Hts-GFP |
actin binding; expressed in epithelium, myoblasts and myotubes |
GFP-fusion (fly TransgeneOme line) |
fTRG585 |
|
4
|
Dlg1-GFP |
epithelial cell junctions, myoblasts and membranes in muscles at all stages |
GFP-fusion (fly TransgeneOme line) |
fTRG502 |
|
4
|
Muscle attachment site
|
βPS-Integrin-GFP |
muscle attachment sites (e.g., starting ≈18 h AFP in indirect flight muscles) |
GFP-knock-in |
- |
|
13
|
Talin-GFP and -mCherry |
muscle attachment sites (e.g., starting ≈18 h AFP in indirect flight muscles) |
GFP-trap (MiMIC line) |
- |
|
3
|
Talin-GFP |
muscle attachment sites (e.g., starting ≈18 h AFP in indirect flight muscles) |
GFP-fusion (fly TransgeneOme line) |
fTRG587 |
|
4
|
Ilk-GFP |
muscle attachment sites (e.g., starting ≈18 h AFP in indirect flight muscles) |
GFP-trap (FlyTrap line) |
Kyoto 110951 (ZCL3111) |
ZCL3111, ZCL3192 |
2
|
Vinc-GFP and -RFP |
muscle attachment sites (e.g., starting ≈18 h AFP in indirect flight muscles) |
GFP-fusion (transgene) |
- |
|
13
|
Tendon
|
sr-GAL4 |
thorax tendon cells, throughout pupal stage |
GAL4 line, enhancer trap |
BL 26663 |
homozygous lethal |
14
|
Muscle and Tendon
|
Duf-GAL4 |
muscles and epithelia, early onset |
GAL4 line |
BL 66682 |
kirre-rP298, founder cell marker |
15
|
UAS-reporters
|
UAS-GFP-Gma |
actin binding |
UAS line |
BL 31776 |
actin binding domain of Moesin fused to GFP |
16
|
UAS-mCherry-Gma |
actin binding |
UAS line |
- |
Gma fused to mCherry |
17
|
UAS-Lifeact-GFP |
actin binding |
UAS line |
BL 35544 |
|
18
|
UAS-Lifeact-Ruby |
actin binding |
UAS line |
BL 35545 |
|
18
|
UAS-CD8-GFP |
membrane binding |
UAS line |
various stocks, e.g.: BL 32184 |
|
19
|
UAS-CD8-mCherry |
membrane binding |
UAS line |
BL 27391 and 27392 |
|
20
|
UAS-palm-mCherry |
membrane binding through palmitoylation |
UAS line |
BL 34514 |
UAS-brainbow |
21
|