Skip to main content
. 2018 Feb 6;(132):57312. doi: 10.3791/57312
Labeled structure Marker Expression and localization Class Stock number Comment Ref.
Muscle Mef2-GAL4 all myoblasts and all muscles at all stages GAL4 line BL 27390 5
1151-GAL4 adult muscle precursors and early myotubes until ≈24 h APF GAL4 line, enhancer trap - 6
Act79B-GAL4 jump muscle upon differentiation GAL4 line - 7
Act88F-GAL4 indirect flight muscles starting ≈14 h APF GAL4 line - 7
Act88F-Cameleon 3.1 indirect flight muscles starting ≈14 h APF Act88F enhancer/ promoter driving Cameleon 3.1 - Ca2+ indicator 8
Act88F-GFP indirect flight muscles starting ≈14 h APF GFP-fusion (fly TransgeneOme line) fTRG78 and fTRG10028 4
Him-nls-GFP adult muscle precursors, nuclear, until ≈24 h APF in indirect flight muscles enhancer/promoter with nls-GFP reporter - 1.5 kb enhancer fragment 9
Mhc-Tau-GFP microtubules in DLM templates and in differentiating muscles enhancer/promoter with Tau-GFP reporter BL 53739 10
βTub60D-GFP microtubules in myotubes (e.g. in indirect flight muscles from ≈14 h AFP, strongly decreasing after ≈48 h APF) GFP-fusion (fly TransgeneOme line) fTRG958 4
Mhc-GFP (weeP26) sarcomeres (thick filament) in all body muscles (e.g. in indirect flight muscles starting from ≈30 h APF) GFP-trap - use heterozygous, labels an isoform subset 11
Sls-GFP sarcomeres (Z-disc) in all body muscles (e.g. in indirect flight muscles starting from ≈30 h APF) GFP-trap (FlyTrap line) - G53, use heterozygous 2
Zasp66-GFP Z-disc in all body muscles GFP-trap (FlyTrap line) BL 6824 ZCL0663 2 12
Zasp52-GFP Z-disc in all body muscles GFP-trap (FlyTrap line) BL 6838 G00189 2 12
Hts-GFP actin binding; expressed in epithelium, myoblasts and myotubes GFP-fusion (fly TransgeneOme line) fTRG585 4
Dlg1-GFP epithelial cell junctions, myoblasts and membranes in muscles at all stages GFP-fusion (fly TransgeneOme line) fTRG502 4
Muscle attachment site βPS-Integrin-GFP muscle attachment sites (e.g., starting ≈18 h AFP in indirect flight muscles) GFP-knock-in - 13
Talin-GFP and -mCherry muscle attachment sites (e.g., starting ≈18 h AFP in indirect flight muscles) GFP-trap (MiMIC line) - 3
Talin-GFP muscle attachment sites (e.g., starting ≈18 h AFP in indirect flight muscles) GFP-fusion (fly TransgeneOme line) fTRG587 4
Ilk-GFP muscle attachment sites (e.g., starting ≈18 h AFP in indirect flight muscles) GFP-trap (FlyTrap line) Kyoto 110951 (ZCL3111) ZCL3111, ZCL3192 2
Vinc-GFP and -RFP muscle attachment sites (e.g., starting ≈18 h AFP in indirect flight muscles) GFP-fusion (transgene) - 13
Tendon sr-GAL4 thorax tendon cells, throughout pupal stage GAL4 line, enhancer trap BL 26663 homozygous lethal 14
Muscle and Tendon Duf-GAL4 muscles and epithelia, early onset GAL4 line BL 66682 kirre-rP298, founder cell marker 15
UAS-reporters UAS-GFP-Gma actin binding UAS line BL 31776 actin binding domain of Moesin fused to GFP 16
UAS-mCherry-Gma actin binding UAS line - Gma fused to mCherry 17
UAS-Lifeact-GFP actin binding UAS line BL 35544 18
UAS-Lifeact-Ruby actin binding UAS line BL 35545 18
UAS-CD8-GFP membrane binding UAS line various stocks, e.g.: BL 32184 19
UAS-CD8-mCherry membrane binding UAS line BL 27391 and 27392 20
UAS-palm-mCherry membrane binding through palmitoylation UAS line BL 34514 UAS-brainbow 21