Table 1.

Early endosome and membrane trafficking proteins

Proteins were detected in all EGF-treated and untreated samples from three independent LC–MS/MS analyses of EEA1-affinity–purified early endosomes. Early endosomes from HeLa cells treated with ±10 ng/ml EGF for 15 min were enriched in a Percoll gradient and affinity-purified using an EEA1 monoclonal antibody. Purified endosomes were eluted in sample buffer, resolved on a 12% SDS-PAGE, stained with Coomassie, and subjected to LC–MS/MS as outlined under “Experimental procedures.” The data are compiled from three independent experiments and quantified using intensity-based absolute quantification (iBAQ). A complete list of proteins can be found at the MassIVE data repository (entry MSV000081692).

Protein abbreviation	Protein name	Molecular mass	Molecular function
		kDa
DNAJC13	DnaJ homolog subfamily C member 13	254	Early endosomal trafficking
EEA1	Early endosome antigen 1	162	Early endosomal trafficking
SNX6	Sorting nexin-6	47	Intracellular trafficking
SNX2	Sorting nexin-2	58	Intracellular trafficking
STX7	Syntaxin-7 (isoform 2)	27	Endocytic trafficking
MYOF	Myoferlin (isoform 6)	233	Endocytic recycling
SCAMP1	Secretory carrier-associated membrane protein 1	38	Intracellular recycling
SCAMP2	Secretory carrier-associated membrane protein 2	37	Intracellular recycling
SCAMP3	Secretory carrier-associated membrane protein 3	38	Intracellular recycling
SNX27	Sorting nexin-27 (Isoform 2)	60	Intracellular recycling
TBC1D5	TBC1 domain family member5 (Isoform 2)	91	Retrograde transport of cargo from endosomes
VP26A	Vacuolar protein sorting-associated protein 26A	38	Component of the retromer cargo-selective complex
VPS35	Vacuolar protein sorting-associated protein 35	92	Component of the retromer cargo-selective complex
CHMP4B	Charged multivesicular body protein 4b	25	Component of ESCRT-III complex
CTSZ	Cathepsin Z	34	Lysosomal cysteine proteinase
STAM2	Cluster of signal transducing adapter molecule 2	58	Down-regulates RTKs via ESCRT-0