Table 3.
Purine labeling with deuterated serine
MEFs were plated in triplicate in 10-cm dishes at a density of 1 × 106 cells per dish and were grown in labeling media for 4 days as described under “Experimental procedures.” Incorporation of the deuterium label into IMP and hypoxanthine (Hyp) was measured by LC-MS. Means of triplicate determinations ± S.D. are shown. IMP + D = IMP + 1 deuteron, Hyp + D = Hyp + 1 deuteron. p values were calculated using two tailed t tests.
| Fraction of total (IMP + IMP + D) |
Fraction of total (Hyp + Hyp + D) |
|||
|---|---|---|---|---|
| IMP | IMP + D | Hyp | Hyp + D | |
| MTHFD1L+/+ | 0.18 ± 0.02 | 0.82 ± 0.02 | 0.22 ± 0.01 | 0.78 ± 0.01 |
| MTHFD1L−/− | 0.90 ± 0.009 | 0.1 ± 0.009 | 0.81 ± 0.03 | 0.19 ± 0.03 |
| p value | 5.87E-06 | 5.87E-06 | 0.00024 | 0.00024 |