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. 2018 Mar 1;293(16):6099–6120. doi: 10.1074/jbc.RA117.000537

Figure 6.

Figure 6.

Phenotypic analysis of PRB958-SC45 chimeric Env glycoproteins. A, schematic representation of PRB958-SC45 chimeric Env glycoproteins. The chimeras contain NL4.3 flanking sequences 5′ and 3′ to the indicated KpnI and BamHI sites, respectively. B, infectivity of WT and mutated viruses for TZM-bl cells. Serially diluted virus-containing supernatants were incubated with TZM-bl cells for 2 days prior to lysis and luciferase assay. The mean RLU ± S.E. obtained from at least three independent experiments is shown. C, gp120 content of virions. Virions were pelleted through a sucrose cushion and then subjected to reducing SDS-PAGE and Western blotting with DV012 (upper panel) and HIVIG (lower panel). gp120-incorporation indices are shown below. The data were obtained from three gels, as indicated. The arrow indicates the position of the 150-kDa marker. D, spreading infection in U87.CD4.CCR5 cells initiated by cell-free and cell-associated virus. Data are representative of two independent experiments. E, spreading infection in PHA-stimulated PBMCs initiated by cell-free and cell-associated virus. Data are representative of two independent experiments.