Figure 6.

Determination of Crh cross-links to MgsA. A, an in vitro chemical cross-linking of MgsA and Crh either alone or in a complex formed in a 1:1 ratio. The samples were treated with the predetermined (Fig. S8) optimal amounts of BS3 using a 1:50 and 1:100 protein/cross-linker ratio. According to the size, bands that represent the complex of MgsA and/or MgsA-Crh were analyzed by LC-MS. B, schematic representation of the intermolecular cross-links determined (see also Table 3 and Figs. S5 and S6).