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Calculated and predicted molecular mass of the various (partially) soluble MgsA forms tested in SEC-MALS
| Experimental mass | Predicted massa | |
|---|---|---|
| kDa | kDa | |
| MgsA | ||
| WT (peak) | 62 | 69.9 (4-mer) |
| 104.8 (6-mer) | ||
| WT (shoulder) | 38 | 34,9 (2-mer) |
| T107R/T111R | Aggregate | 35.1 (2-mer) |
| Q62R/A65R | 34.1 | 35.1 (2-mer) |
| Q62R | 41.3 | 34.9 (2-mer) |
| A93W/L97W | 34.4 | 35.3 (2-mer) |
| Crh | ||
| WT | 20.5/19.8 | 21.0 (2-mer) |
| 10.3/9.6 | 10.5 (1-mer) | |
a All masses were calculated via ExPASy ProtParam as expressed, including tags.
