Figure 6.
Activation of the macrophages in co-culture with islets via TLR4. Peritoneal macrophages (1 × 105 cells) were co-cultured with isolated pancreatic islets (50 islets) in a medium containing BSA (0.5%) in the presence/absence of palmitate. A and B, mRNA expression levels in macrophages co-cultured with islets for 24 h. Horizontal bars, mean values. **, p ≤ 0.01 (n = 5). C, mRNA expression levels in macrophages co-cultured with islets in the presence of the TLR4 inhibitory peptide VIPER or the control peptide CP7 for 24 h. Horizontal bars, mean values. *, p ≤ 0.05 (n = 5).