Figure 1.

Yeast surface display screen. A, fluorescence profile of yeast surface display library expressing putative tick evasins incubated with streptavidin-AF647 alone. The red outlined box indicates the sorting gate. B, yeast surface display library incubated with biotinylated CCL8 plus streptavidin-AF647. The sorting gate was identified from the negative control and was used to sort CCL8-binding yeast from the library. C, second round sorting of CCL8-binding yeast identified above. The proportions of cells within the sorting gate are indicated as a percentage. D, binding of P672 to CCL8. Yeast expressing P672 are shown in red, and control yeast bearing empty vector surface display plasmid are shown in blue. The y axis shows cell count and x axis the fluorescence intensity on a log₁₀ scale. The proportion of cells exceeding background is indicated as a percentage.