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Analysis of glycosylated and deglycosylated P672 by SDS-PAGE. The gel is stained with colloidal Coomassie Blue. Shown are molecular mass markers (kDa, lane 1), N-terminally tagged P672 protein (lane 2), C-terminally tagged P672 protein (lane 3), and N-terminally tagged P672 protein (lane 4), partially deglycosylated using PNGaseF and EndoF1. All proteins were purified using nickel affinity followed by size exclusion chromatography.
