Figure 8.

Barrier and metabolite responses of chemically ATP-depleted cells to hypoxanthine. A, relative transepithelial resistance measurements of control, 2-deoxyglucose (20 mm)–treated, and 2-deoxyglucose + hypoxanthine (1 mm)–treated T84 cells; n = 3, t = 2 h. B, metabolite responses of 2-deoxyglucose–treated cells supplemented with hypoxanthine as measured by HPLC. Statistics are shown for 2-deoxyglucose-treated samples only; n = 3, t = 2 h. C, total available energy response of experiments depicted in B. Data are presented as mean ± S.D. (error bars). Total available energy = PCr + ATP + (0.5 × ADP); ***, p < 0.001.