Figure 7.

TMEPAI overexpression leads to myeloma cell apoptosis in association with c-Maf degradation. A, the TMEPAI expression level was negatively associated with the overall survival of MM patients based on the prognostic gene analysis (PROGgene database, http://watson.compbio.iupui.edu/chirayu/proggene/database/index.php).⁵ B, TMEPAI is down-regulated in myeloma cells based the DNA microarray analysis on the typical 60 cancer cell lines from National Cancer Institute (NCI 60 cell line). C, TMEPAI expression was analyzed by RT-PCR in bone marrow cells from healthy donors (normal bone marrow (NBM)) and MM patients. D, the densitometric analysis of TMEPAI from C. E, myeloma cell lines RPMI-8226 and LP1 were infected with lentiviral TMEPAI for 96 h, followed by immunoblotting assay against specific antibodies. F, myeloma cell lines were infected with lentiviral TMEPAI for 96 h followed by annexin V/propidium iodide staining and flow cytometric analysis. plvx, the lentivirus without TMEPAI gene.