Cryo-EM images of HIV-1 NL4-3 WT (IN TT 124/125) and HIV-1 NL4-3 polymorphic IN AA 124/125 virus particles treated in the presence or absence of MUT-A.
Red arrows indicate the formation of eccentric condensates; blue arrows indicate normal conical cores; and green arrows show nonconical cores. A, NL4-3 IN AA 124/125 polymorphic virus produced from 293T cells, transfected with pNL4-3 IN AA 124/125, in the presence of DMSO and in the absence of MUT-A (negative control). B, NL4-3 WT (IN TT 124/125) virus produced in the presence of 1 μm MUT-A (positive control showing eccentric condensate). C, NL4-3 IN AA 124/125 polymorphic virus produced in the presence of 5 μm MUT-A. Scale bars, 100 nm.