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Surface plasmon resonance binding of the collagen-binding domain of GP6–CBD to type IV collagen from WT or P3H2 koPFHR-9 cells. Injection was from 5 to 35 s, and dissociation was from 35 to 65 s. Concentration for all injections was 35 μg/ml (1.75 μm). Each curve is an average of three injections. Response was normalized for the amount of bound collagen IV.
