Figure 6.
Steady-state affinities of GP6–CBD for type IV collagen from surface plasmon resonance data. A, data from GP6–CBD binding to P3H2 KO PFHR-9 type IV collagen. B, data from GP6–CBD binding to WT PFHR-9 type IV collagen. Three replicate responses are shown for each of four concentrations as red dots. Steady-state 1:1 model fit is shown as a black line. Response units for each data point are an average of the response from t = 20 s to t = 30 s for each 30-s injection. The modeled Kd for P3H2 KO type IV collagen was 2.1 and 84 μm for the WT.