Table 1.
Amino acid analysis quantitation of hydroxyproline and hydroxylysine
Experimental concentration was derived from peak integration and the average of three runs. Experimental concentrations for each individual amino acid were normalized to the total Pro + 3-Hyp + 4-Hyp or Lys + Lys-OH content. The average occupancy is expressed at a fraction of the normalized experimental concentration divided by the number of corresponding motifs (i.e. GPP for 3-Hyp) present in a type IV collagen α1 α1 α2 trimer lacking the N-terminal signaling peptide.
| Amino acid | WT |
P3H2 KO |
||
|---|---|---|---|---|
| Experimental composition | Normalized composition | Experimental composition | Normalized composition | |
| 3-Hyp | 31.5 (3.35%) | 30.6/109 (GPP) | 4.0 (0.46%) | 4.2/109 (GPP) |
| 4-Hyp | 587.1 (62.4%) | 571.0/591 (GXP) | 505.1 (58.5%) | 535.3/591 (GXP) |
| Pro | 321.9 (34.2%) | 313.0/915 (P) | 354.8 (41.1%) | 376.1/915 (P) |
| Pro + 3-Hyp + 4-Hyp | 940.5 (100%) | 915/915 (P) | 863.8 (100%) | 915/915 (P) |
| Lys | 46.5 (17.6%) | 45.2/257 (K) | 68.6 (25.5%) | 65.5/257 (K) |
| Lys-OH | 217.6 (82.4%) | 211.8/202 (GXK) | 200.8 (74.5%) | 191.5/202 (GXK) |
| Lys + Lys-OH | 264.1 (100%) | 257/257 (K) | 269.4 (100%) | 257/257 (K) |