Table 2.
Level of modification of individual 3-Hyp sites in type IV collagen from WT or P3H2 KO (6A6) PFHR-9 cells
Modification levels are based on MS1 spectra, which can be found with the corresponding figure (i.e. 197 COL4A2 MS1 and MS2 data are found in Fig. S29). Peak occupancies are estimated to the nearest 5% based on MS1 peak height. F, figure; SF, supplemental figure; NA, not applicable.
| Residue no. | Occupancy |
Figure |
||
|---|---|---|---|---|
| WT | P3H2 KO | WT | P3H2 KO | |
| % | % | |||
| 137 COL4A1 | 0 | 0 | SF9 | SF23 |
| 149 COL4A1 | 20 | 0 | SF9 | SF23 |
| 198 COL4A1 | 100 | No data | F3/ (MS2 SF36) | NA |
| 204 COL4A1 | 60 | 0 | F3/ (MS2 SF36) | F3/ (MS2 SF35) |
| 207 COL4A1 | 60 | 0 | F3/ (MS2 SF36) | F3/ (MS2 SF35) |
| 210 COL4A1 | 60 | 0 | F3/ (MS2 SF36) | F3/ (MS2 SF35) |
| 213 COL4A1 | 60 | 0 | F3/ (MS2 SF36) | F3/ (MS2 SF35) |
| 239 COL4A1 | No data | 0 | NA | SF37 |
| 330 COL4A1 | 0 | 0 | SF10 | SF24 |
| 372 COL4A1 | No data | 0 | NA | SF25 |
| 478 COL4A1 | 60 | 0 | SF11 | SF26 |
| 484 COL4A1 | 0 | 0 | SF11 | SF26 |
| 587 COL4A1 | 0 | No data | SF12 | NA |
| 647 COL4A1 | 15 | 0 | SF13 | SF27 |
| 1214 COL4A1 | 8–35 | No data | SF40 | NA |
| 1345 COL4A1 | 25 | No data | SF14 | NA |
| 1348 COL4A1 | 55 | No data | SF14 | NA |
| 1367 COL4A1 | 0 | No data | SF15 | NA |
| 1424 COL4A1 | 80 | No data | SF16a/b | NA |
| 1436 COL4A1 | 15 | 0 | SF16a/b | SF28 |
| 74 COL4A2 | 80 | 15 | SF38 | SF38 |
| 197 COL4A2 | No data | 0 | NA | SF29 |
| 343 COL4A2 | 60 | No data | SF17 | NA |
| 386 COL4A2 | 50 | 0 | SF18 | SF30 |
| 389 COL4A2 | 5 | No data | SF18 | NA |
| 586 COL4A2 | 0 | 0 | SF19 | SF31 |
| 594 COL4A2 | 0 | No data | SF19 | NA |
| 638 COL4A2 | 40 | 0 | SF20 | SF32 |
| 644 COL4A2 | 70 | 0 | SF20 | SF32 |
| 647 COL4A2 | 15 | 0 | SF20 | SF32 |
| 728 COL4A2 | 65 | No data | SF39 | NA |
| 970 COL4A2 | 60 | 0 | SF21 | SF33 |
| 973 COL4A2 | 0 | 0 | SF21 | SF33 |
| 1419 COL4A2 | 60 | 0 | F4/ (MS2 SF22) | F4/ (MS2 SF34) |