Figure 4.

Growth of E. coli strain ΔfumACB expressing PfFH on different carbon sources. a–f, the growth of ΔfumACB strains expressing either PfFHFL (FL) or PfFHΔ40 (Δ40) or PfFHΔ120 (Δ120) were tested on meso-tartrate (a), mesaconate (b), d-tartrate (c), l-tartrate (d), itaconate (e), and glucose-containing minimal medium (f). The plates were scored after 48 h of incubation at 37 °C. ΔfumACB strain containing just pQE30 (E) was used as a control. The experiment was repeated three times, and the images correspond to one of the replicates. The glucose-containing plate served as a control for the number of cells plated across the different constructs.