Table 2.

Kinetic parameters of PfFHΔ40 and other class I FH

The units for K_m, k_cat, and k_cat/K_m are mm, s⁻¹, and s⁻¹ m⁻¹, respectively.

Organism/enzyme name	Substrate	Km	kcat	kcat/Km	Refs.
P. falciparum
PfFHΔ40	Fumarate	2.6 ± 0.3	182 ± 8	7.0 × 104	This study
	Malate	1.2 ± 0.1	159 ± 9	1.3 × 105
	Mesaconate	3.2 ± 0.3	60 ± 2	1.9 × 104
T. cruzi
TcFHca	Fumarate	0.8 ± 0.2b	400 ± 100	8.2 × 106c	22
	Malate	2.5 ± 0.6b	290 ± 40	1.3 × 106c
TcFHmd	Fumarate	1.5 ± 0.4	2300 ± 500	1.5 × 106
	Malate	2.8 ± 0.2	1050 ± 40	0.4 × 106
E. colie
fumA	Fumarate	0.09 ± 0.02	617	6.6 × 106	14
	Malate	0.40 ± 0.05	352	8.7 × 105
	Mesaconate	0.22 ± 0.02	56	2.5 × 105
fumB	Fumarate	0.21 ± 0.03	655	3.1 × 106
	Malate	0.78 ± 0.13	290	3.7 × 105
	Mesaconate	0.10 ± 0.01	58	5.8 × 105
L. major
LMFH-1f	Fumarate	2.5 ± 0.4	28.3 ± 4.7	1.1 × 104	21
	Malate	2.3 ± 0.3	12.9 ± 1.3	5.6 × 103
LMFH-2g	Fumarate	5.7 ± 1.4	204.2 ± 52.1	3.6 × 104
	Malate	12.6 ± 2.7	151.4 ± 20.5	1.2 × 104
P. furiosuse
FH	Fumarate	0.34	1101	3.2 × 106	69
	Malate	0.41	1514	3.7 × 106
P. thermop.h
MmcBCi	Fumarate	0.43	219	5.1 × 105	8
	Malate	0.59	25.2	4.3 × 104
B. xenovoranse
Bxe_A3136j	Fumarate	0.10 ± 0.01	296	2.8 × 106	10
	Malate	0.28 ± 0.02	118	3.98 ×105
	Mesaconate	0.03 ± 0.01	117	3.6 × 106

^a This is cytosolic T. cruzi FH.

^b TcFHc exhibits cooperativity and hence k_0.5 values are reported. The value of Hill coefficient is 1.4 for both substrates.

^c The values reported are k_cat/k^h_0.5 with units being s⁻¹ m^−1.4.

^d This is mitochondrial T. cruzi FH.

^e The k_cat values provided in the table for FH from E. coli, P. furiosus, and B. xenovorans are calculated from the V_max values reported in the reference provided.

^f LMFH-1 is mitochondrial L. major FH.

^g LMFH-2 is cytosolic L. major FH.

^h P. thermopropionicum is abbreviated as P. thermop.

ⁱ Putative FH in P. thermopropionicum has been annotated as MmcBC (8).

^j UniProt id is for FH from B. xenovorans (10).