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Egg-laying profile (A) and total brood size (B) of WT (n = 27), pamn-1 (n = 35), pgal-1 (n = 32), pghm-1 (n = 22), and pghm-1;pamn-1 (n = 21) mutant worms. The single mutants do not show any severe defects in egg-laying behavior nor a difference in total brood size. Inactivation of both pghm-1 and pamn-1, however, results in a severely diminished brood size that is reduced by 42% compared with WT (*, p < 0.05; **, p < 0.01; ***, p < 0.001).
