The growth of bacteria in a zinc-deficient environment.
A, the absorbance at 600 nm of WT S.
pneumoniae (D39) and ΔadcA
ΔadcAII mutant strains cultured in C+Y medium
containing TPEN at different final concentrations for 8 h. B,
the growth curves (left) of D39 strains grown in C+Y medium
containing 20 μm TPEN and corresponding growth rate constants
(right) calculated for WT, ΔadcA
ΔadcAII, and ΔadcA
ΔadcAII with the N-lobe, C-lobe, and full-length
adcA, respectively, and error bars
represent S.D. AU, absorbance units.