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. Author manuscript; available in PMC: 2018 Apr 23.
Published in final edited form as: Nat Chem. 2018 Jan 8;10(3):363–371. doi: 10.1038/nchem.2919

Figure 4. Structure of lipid II showing binding sites for antibiotics and mass spectra recorded after addition of antibiotics to MurJ and to the Mur:lipid II complex.

Figure 4

Regions of the lipid II structure are coloured according to the vancomycin and ramoplanin binding sites (36–38) (orange and yellow respectively). a Mass spectra of MurJ with vancomycin shows the 9+ charge state of MurJ and the absence of any appreciable adduct peaks corresponding to bound vancomycin. The mass spectra of MurJ and lipid II (at final concentrations of 5 μM and 3 μM respectively) confirm complex formation and addition of vancomycin (3 μM and 7 μM) leads to formation of the ternary complex. b Analogous spectra were recorded for ramoplanin. Addition of ramoplanin at low concentrations (1.5 μM and 3.0 μM) lead to disappearance of the MurJ:lipid II complex. All experiments were performed three times and spectra shown are representative.