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. Author manuscript; available in PMC: 2018 Apr 23.
Published in final edited form as: Nat Chem. 2018 Jan 8;10(3):363–371. doi: 10.1038/nchem.2919

Figure 6. Mass spectra reveal the effect of increasing cardiolipin concentration on lipid II binding to MurJ.

Figure 6

In the absence of cardiolipin the MurJ:lipid II (5 μM:3 μM) complex is formed (relative intensity ~45% of the MurJ peak). As the concentration of cardiolipin is increased (from 2.5 μM to 10 μM) the lipid II binding peak decreases and the MurJ:CDL complex predominates. All experiments were performed three times and standard deviations are calculated from the average of the five observed charge states in three independent experiments. Error bars represent standard deviations (n=3).