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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Mucosal Immunol. 2017 Dec 20;11(3):681–692. doi: 10.1038/mi.2017.105

Figure 6. The mucosal response to sFliC contributes to the systemic antibody response.

Figure 6

Irf4fl/fl (black) or Cd11c-cre.Irf4fl/fl (white) mice were either non-immunized or sFliC prime-boosted. (a) ELISPOT analysis of sFliC IgG and IgA responses in the BM. Number of spot-forming units (SFU) per 5x105 cells (graphs) and representative pictures of wells (lower panels). Data are shown as mean +SD (n=12 mice/group) of three independent experiments pooled together. ***p < 0.0001, by two-way ANOVA. (b) Cd11c-cre.Irf8fl/fl (white) or Irf8fl/fl control mice (black) were either non-immunized or sFliC-primed boosted. ELISPOT analysis of sFliC IgG and IgA responses in the BM. Data are shown as mean +SD (n=8 mice/group) of two independent experiments pooled together. *p < 0.01, by two way ANOVA. (c) WT mice were non-immunized or sFliC primed-boosted. BM CD138+ cells were intracellularly stained with sFliC-biotynilated, expression of α4β7 is shown in sFliC+ and sFliC- CD138+ cells by FACS and (d) cytospins show α4β7 (brown) and sFliC (blue) in pre-enriched CD138+ cells. Scale bar indicates 20 μm. Representative plots and photomicrographs (n=4 mice/group) from 2 independent experiments. (e) Serum anti-sFliC IgG and IgA evaluated by ELISA. Data are shown as mean +SD (n=12 mice/group) and are representative of three independent experiments pooled together. ***p ≤ 0.0001, by two way ANOVA.