Table S1.

Immunization protocol

Formulation	Antigen dose (μg)	Volume (μL)	Immunization schedule (days)	Serum (days)	Saliva, vaginal fluid (days)	Immunocytes (splenocytes, macrophages) (days)
PBS	0	20	1, 15, 29	14, 28, 42	42	42
Ova	20	20	1, 15, 29	14, 28, 42	42	42
Ova + AL	20	20	1, 15, 29	14, 28, 42	42	42
Ova + CS	20	20	1, 15, 29	14, 28, 42	42	42
Ova + O-HTCC	20	20	1, 15, 29	14, 28, 42	42	42
CS + O-HTCC	0	20	1, 15, 29	14, 28, 42	42	42
Ova/CS/O-HTCC	20	20	1, 15, 29	14, 28, 42	42	42

Abbreviations: CS, curdlan sulfate; O-HTCC, O-(2-hydroxyl)propyl-3-trimethyl ammonium chitosan chloride; Ova, ovalbumin; AL, Alhydrogel.