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. Author manuscript; available in PMC: 2019 Apr 19.
Published in final edited form as: Mol Cell. 2018 Apr 19;70(2):242–253.e6. doi: 10.1016/j.molcel.2018.03.016

Figure 4. The ERAD of an unstable, aggregation-prone substrate is Hsp104-dependent.

Figure 4

The stabilities of Q394X in the indicated wild type and mutant strains were determined by cycloheximide chase analyses at 37°C. (A) The yeast cytosolic Hsp110, Sse1; N = 3 independent experiments, +/− SEM. (B) The yeast cytosolic disaggregase, Hsp104; N = 3–6 independent experiments, +/− SEM; black asterisks apply to the HSP104 vs hsp104Δ comparison, and the red asterisk applies to the HSP104 vs HSP104 OE comparison. In all panels, *P < 0.05, **P < 0.01, ***P < 0.001.