The stabilities of Q394X in the indicated wild type and mutant strains were determined by cycloheximide chase analyses at 37°C. (A) The yeast cytosolic Hsp110, Sse1; N = 3 independent experiments, +/− SEM. (B) The yeast cytosolic disaggregase, Hsp104; N = 3–6 independent experiments, +/− SEM; black asterisks apply to the HSP104 vs hsp104Δ comparison, and the red asterisk applies to the HSP104 vs HSP104 OE comparison. In all panels, *P < 0.05, **P < 0.01, ***P < 0.001.