Antibodies |
12CA5 anti-HA (mouse) |
Roche Applied Science |
Cat# 11583816001 |
Anti-HA-Peroxidase High Affinity (3F10, Rat) |
Roche Diagnostics |
Cat# 12013819001 |
Anti-glucose-6-phosphate-dehydrogenase (Rabbit) |
Sigma Aldrich |
Cat# A9521 |
Anti-rabbit, HRP-linked antibody (Donkey) |
Cell Signaling Technology |
Cat# 7074S |
Anti-mouse, HRP-linked antibody (Horse) |
Cell Signaling Technology |
Cat# 7076S |
Anti-Kar2 primary antibody (Rabbit) |
Brodsky Lab |
N/A |
Anti-rabbit, Alexa 568 (Goat) |
Thermo Fisher Scientific |
Cat# A-11011 |
Anti-mouse, Alexa 488 (Goat) |
Thermo Fisher Scientific |
Cat# A-11001 |
Anti-Sec61 (Rabbit) |
Brodsky Lab |
N/A |
Anti-ubiquitin (P4D1, Mouse) |
Santa Cruz Biotechnology |
Cat# sc-8017 |
Anti-Hsp104 (Rabbit) |
Glover Lab |
N/A |
Biological Samples |
Ubiquitin from bovine erythrocytes |
Sigma Aldrich |
Cat# U6253 |
Chemicals, Peptides, and Recombinant Proteins |
Cycloheximide |
Sigma Aldrich |
Cat# C7698 |
SuperSignal Chemiluminescence |
Thermo Fisher Scientific |
Cat# 34077 |
Z-Leu-Leu-Leu-al (MG132) |
Selleckchem |
Cat# S2619 |
Zymolyase 20T |
MP Biomedicals |
Cat# 320921 |
Dodecyl-beta-D-Maltoside (DDM) |
Calbiochem |
Cat# D310 |
cOmplete™, EDTA-free Protease Inhibitor Cocktail |
Roche |
Cat# 11836170001 |
Apyrase |
Sigma Aldrich |
Cat# A7646 |
Leupeptin |
Sigma Aldrich |
Cat# L9783 |
Pepstatin A |
Sigma Aldrich |
Cat# P5318 |
N-ethylmaleimide (NEM) |
Sigma Aldrich |
Cat# E1271 |
Poly-L-lysine, hydrobromide |
Sigma Aldrich |
Cat# P2636 |
4′,6-Diamidino-2-phenylinadole (DAPI) |
Sigma Aldrich |
Cat# D8417 |
Critical Commercial Assays |
EasyTag™ EXPRESS 35S Protein Labeling Mix |
PerkinElmer Life Sciences |
Cat# NEG772007MC |
BioRad Protein Assay Reagent |
Bio-Rad |
Cat# 500-0006 |
Gibson Assembly Master Mix |
New England Biolabs |
Cat# E2611S |
Deposited Data |
http://dx.doi.org/10.17632/zw2ff8yyg7.2 |
|
|
Experimental Models: Organisms/Strains |
See Table S1
|
Oligonucleotides |
See Table S2
|
Recombinant DNA |
See Table S3
|
Software and Algorithms |
Quantity One software |
Bio-Rad Laboratories |
CAT# 1709600 |
ImageJ 1.48V |
Wayne Rasband, NIH |
www://imagej.nih.gov/ij |
Pymol version 1.7.4.5 Edu |
The PyMOL Molecular Graphics System, Schrodinger, LLC. |
http://www.pymol.org |
Excel 2013 |
Microsoft |
https://www.microsoftstore.com |
Sigma Plot 11.0 |
Systat |
https://systatsoftware.com/products/sigmaplot/ |
Other |
Prolong Gold Antifade Reagent |
Molecular probes |
Cat# P10144
|
Anti-HA conjugated resin |
Roche |
Cat# 11815016001 |
Iodine-125 Radionuclide |
PerkinElmer |
Cat# NEZ033005MC |
Sephacryl S-300HR |
GE Healthcare Life Sciences |
Cat# 17059910 |