REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
12CA5 anti-HA (mouse)	Roche Applied Science	Cat# 11583816001
Anti-HA-Peroxidase High Affinity (3F10, Rat)	Roche Diagnostics	Cat# 12013819001
Anti-glucose-6-phosphate-dehydrogenase (Rabbit)	Sigma Aldrich	Cat# A9521
Anti-rabbit, HRP-linked antibody (Donkey)	Cell Signaling Technology	Cat# 7074S
Anti-mouse, HRP-linked antibody (Horse)	Cell Signaling Technology	Cat# 7076S
Anti-Kar2 primary antibody (Rabbit)	Brodsky Lab	N/A
Anti-rabbit, Alexa 568 (Goat)	Thermo Fisher Scientific	Cat# A-11011
Anti-mouse, Alexa 488 (Goat)	Thermo Fisher Scientific	Cat# A-11001
Anti-Sec61 (Rabbit)	Brodsky Lab	N/A
Anti-ubiquitin (P4D1, Mouse)	Santa Cruz Biotechnology	Cat# sc-8017
Anti-Hsp104 (Rabbit)	Glover Lab	N/A
Biological Samples
Ubiquitin from bovine erythrocytes	Sigma Aldrich	Cat# U6253
Chemicals, Peptides, and Recombinant Proteins
Cycloheximide	Sigma Aldrich	Cat# C7698
SuperSignal Chemiluminescence	Thermo Fisher Scientific	Cat# 34077
Z-Leu-Leu-Leu-al (MG132)	Selleckchem	Cat# S2619
Zymolyase 20T	MP Biomedicals	Cat# 320921
Dodecyl-beta-D-Maltoside (DDM)	Calbiochem	Cat# D310
cOmplete™, EDTA-free Protease Inhibitor Cocktail	Roche	Cat# 11836170001
Apyrase	Sigma Aldrich	Cat# A7646
Leupeptin	Sigma Aldrich	Cat# L9783
Pepstatin A	Sigma Aldrich	Cat# P5318
N-ethylmaleimide (NEM)	Sigma Aldrich	Cat# E1271
Poly-L-lysine, hydrobromide	Sigma Aldrich	Cat# P2636
4′,6-Diamidino-2-phenylinadole (DAPI)	Sigma Aldrich	Cat# D8417
Critical Commercial Assays
EasyTag™ EXPRESS 35S Protein Labeling Mix	PerkinElmer Life Sciences	Cat# NEG772007MC
BioRad Protein Assay Reagent	Bio-Rad	Cat# 500-0006
Gibson Assembly Master Mix	New England Biolabs	Cat# E2611S
Deposited Data
http://dx.doi.org/10.17632/zw2ff8yyg7.2
Experimental Models: Organisms/Strains
See Table S1
Oligonucleotides
See Table S2
Recombinant DNA
See Table S3
Software and Algorithms
Quantity One software	Bio-Rad Laboratories	CAT# 1709600
ImageJ 1.48V	Wayne Rasband, NIH	www://imagej.nih.gov/ij
Pymol version 1.7.4.5 Edu	The PyMOL Molecular Graphics System, Schrodinger, LLC.	http://www.pymol.org
Excel 2013	Microsoft	https://www.microsoftstore.com
Sigma Plot 11.0	Systat	https://systatsoftware.com/products/sigmaplot/
Other
Prolong Gold Antifade Reagent	Molecular probes	Cat# P10144
Anti-HA conjugated resin	Roche	Cat# 11815016001
Iodine-125 Radionuclide	PerkinElmer	Cat# NEZ033005MC
Sephacryl S-300HR	GE Healthcare Life Sciences	Cat# 17059910