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. 2018 Apr 23;16(4):e2005129. doi: 10.1371/journal.pbio.2005129

Fig 5. Virulence factor mutants identified by iPool-Seq cause reduced disease symptoms on maize.

Fig 5

(a) Disease rating of insertional mutant strains 7 dpi. Mean standard deviation of relative counts from 3 replicates are displayed. Only positive error bars are shown. p-Values were calculated by Fishers exact test. Multiple testing correction was done by Benjamini-Hochberg algorithm. **** p < 0.0001. (S2 Data) (b) Growth assay of insertion mutants on (A) Cm-medium, or Cm-medium supplemented with (B) 75 μg/mL Calcofluor (cell wall stress), (C) 45 μg/mL Congo red (cell wall stress), and (D) Charcoal (b-filament inducing). (c) Confocal microscopy of maize infected with indicated insertional mutant strains 7 dpi. Infected plant tissue was stained with propidium iodide (red) and fungal hyphae with lectin binding WGA-AF488 (green). One representative picture of 9 infected plants is shown. Cm-medium, control Complete medium; dpi, days post infection; iPool-Seq, insertion Pool-Sequencing; ref, reference; wt, wild-type.