FIG. 2.

Enhancement of transcriptional co-activator with PDZ-binding motif (TAZ) in Activin A–treated human embryonic stem (hES) cells by ethacridine. Dose-dependent increase in TAZ and its translocation into the nucleus after treatment is shown here. (A) Representative qRT-PCR analysis for TAZ of ethacridine-treated endoderm cells derived from hES cells by Activin A after two days at 37°C. All data are expressed as mean ± SEM and represent one of three separate experiments. (B) The nuclear translocation of TAZ detected by immunostaining. Nuclear localization of TAZ after 10 μM of ethacridine treatment is indicated by TAZ staining as the green color located in the nucleus in the lower panel in comparison to the control (untreated) cells where TAZ is located in the cytoplasm as shown in the upper panel. Scale = 20 μm.