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. 2017 Feb 1;27(2):292–299. doi: 10.1089/thy.2016.0264

FIG. 3.

FIG. 3.

Enhancement of PAX8 and NKX2-1 in Activin A–treated hES cells by ethacridine. (A) Representative qRT-PCR analysis for thyroid transcription factors PAX8 and NKX2-1 on ethacridine-treated endoderm cells derived from hES cells by Activin A. Data are expressed as mean ± SEM and represent one of three separate experiments. Statistical analysis by analysis of variance (ANOVA) gave a p-value of *p < 0.0001 for NKX2-1 and PAX8 in each of the Activin A + ethacridine-treated cells compared with only Activin A–treated cells. (B–G) Immunostaining of PAX8 (B, staining as green), NKX2-1 (D, staining as red), NKX2-1 (red), and TAZ (green) (F) after treating with 10 μM of ethacridine. Cells without ethacridine treatment served as control and are shown in the right panels (C, E, and G). Scale = 20 μm.