FIG. 5.

Demonstration of differentiated hES cell thyroid cell function. Differentiated hES cells were exposed to Na¹²⁵I as described in the Methods, and the radioactive uptake (A) and protein bound ¹²⁵I (B) in the differentiated thyroid cells was measured with and without thyrotropin (TSH; 0.01 and 0.1 mIU/mL). Sodium perchlorate was used to inhibit the NIS activity. Undifferentiated hES cells were used as control. Statistical analysis was performed with ANOVA. p-Values: *p < 0.01 and ***p < 0.0001 in the TSH-treated groups compared to untreated controls, and #p < 0.0001 in the 100 μIU/mL TSH-treated cells compared to 100 μIU/mL TSH + sodium perchlorate–treated cells.