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. 2017 Dec 30;58(4):E308–E314. doi: 10.15167/2421-4248/jpmh2017.58.4.711

Tab. I.

PCR primers and cycling parameters for genes presented in this study.

Gene target Primer/sequence(5’-3’) PCR condition PCR product (bp) Reference
chp F-GAAAAAGAAATTAGCAACAACAG
R-CATAAGATGATTTAGACTCTCC
30 sec 95ºC,50 sec 48ºC,1min 72ºC 410 [18]
sak F-AAGGCGATGACGCGAGTTAT
R-GCGCTTGGATCTAATTCAAC
30 sec 94 ºC,30 sec 50 ºC,1min 72 ºC 223 [14]
sea F-AGATCATTCGTGGTATAACG
R-TTAACCGAAGGTTCTGTAGA
30 sec 94 ºC,30 sec 50 ºC,1min 72 ºC 408 [14]
sep F-AATCATAACCAACCGAATCA
R-TCATAATGGAAGTGCTATAA
30 sec 94 ºC,30 sec 50 ºC,1min 72 ºC 500 [14]
scn F-AGCACAAGCTTGCCAACATCG
R-TTAATATTTACTTTTTAGTGC
30 sec 94 ºC,30 sec 49 ºC,1min 72 ºC 258 [14])
icaA F- TCTCTTGCAGGAGCAATCAA
R-TCAGGCACTAACATCCAGCA
1 min 95 ºC,45 sec 60 ºC,1min 72 ºC 188 [28]
icaB F- ATGGCTTAAAGCACACGACGC
R- TATCGGCATCTGGTGTGACAG
1 min 95 ºC,45 sec 61 ºC,1min 72 ºC 526 [29]
icaC R- CTCTCTTAACATCATTCCGACGCC
F- ATCATCGTGACACACTTACTAACG
1 min 95 ºC,45 sec 63 ºC,1min 72 ºC 1013 [29]
icaD F -GAACCGCTTGCCATGTGTTG
R- GCTTGACCATGTTGCGTAACC
1 min 95 ºC,45 sec 61 ºC,1min 72 ºC 483 [53]
mecA F-TCC AGA TTA CAA CTT CAC CAG G
R-CCA CTT CAT ATC TTG TAA CG
1 min 95 ºC,45 sec 56 ºC,1min 72 ºC 162 [26]
nuc F-GCGATTGATGGTGATACGGTT
R-AGCCAAGCCTTGACGAACTAAAGC
1 min 95 ºC,45 sec 60 ºC,1min 72 ºC 279 [30]