Tab. I.
Gene target | Primer/sequence(5’-3’) | PCR condition | PCR product (bp) | Reference |
---|---|---|---|---|
chp | F-GAAAAAGAAATTAGCAACAACAG R-CATAAGATGATTTAGACTCTCC |
30 sec 95ºC,50 sec 48ºC,1min 72ºC | 410 | [18] |
sak | F-AAGGCGATGACGCGAGTTAT R-GCGCTTGGATCTAATTCAAC |
30 sec 94 ºC,30 sec 50 ºC,1min 72 ºC | 223 | [14] |
sea | F-AGATCATTCGTGGTATAACG R-TTAACCGAAGGTTCTGTAGA |
30 sec 94 ºC,30 sec 50 ºC,1min 72 ºC | 408 | [14] |
sep | F-AATCATAACCAACCGAATCA R-TCATAATGGAAGTGCTATAA |
30 sec 94 ºC,30 sec 50 ºC,1min 72 ºC | 500 | [14] |
scn | F-AGCACAAGCTTGCCAACATCG R-TTAATATTTACTTTTTAGTGC |
30 sec 94 ºC,30 sec 49 ºC,1min 72 ºC | 258 | [14]) |
icaA | F- TCTCTTGCAGGAGCAATCAA R-TCAGGCACTAACATCCAGCA |
1 min 95 ºC,45 sec 60 ºC,1min 72 ºC | 188 | [28] |
icaB | F- ATGGCTTAAAGCACACGACGC R- TATCGGCATCTGGTGTGACAG |
1 min 95 ºC,45 sec 61 ºC,1min 72 ºC | 526 | [29] |
icaC | R- CTCTCTTAACATCATTCCGACGCC F- ATCATCGTGACACACTTACTAACG |
1 min 95 ºC,45 sec 63 ºC,1min 72 ºC | 1013 | [29] |
icaD | F -GAACCGCTTGCCATGTGTTG R- GCTTGACCATGTTGCGTAACC |
1 min 95 ºC,45 sec 61 ºC,1min 72 ºC | 483 | [53] |
mecA | F-TCC AGA TTA CAA CTT CAC CAG G R-CCA CTT CAT ATC TTG TAA CG |
1 min 95 ºC,45 sec 56 ºC,1min 72 ºC | 162 | [26] |
nuc | F-GCGATTGATGGTGATACGGTT R-AGCCAAGCCTTGACGAACTAAAGC |
1 min 95 ºC,45 sec 60 ºC,1min 72 ºC | 279 | [30] |