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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Nat Chem Biol. 2017 Dec 18;14(2):163–170. doi: 10.1038/nchembio.2538

Figure 3. THAL-SNS-032 exhibits CRBN-dependent anti-proliferative and pro-apoptotic effects.

Figure 3

a. Cell viability IC50s for THAL-SNS-032, SNS-032, or NVP-2 on wild-type and CRBN−/− MOLT4 after 72 hours as approximated by using CellTiter Glo (data are presented as means ± s.d. (n=4) biological replicates). b. Immunoblot of proteins shown for the indicated times after treatment with THAL-SNS-032 at 250 nM in wildtype MOLT4 cells. c. Immunoblot of proteins shown for the indicated times after treatment with 250nM NVP-2 in wildtype MOLT4 cells. d. Immunoblot of proteins shown for the indicated times after treatment with 250nM SNS-032 in wildtype MOLT4 cells. e. Viability effect of THAL-SNS-032, SNS-032 or NVP-2 of MOLT4 cells at 250nM concentration of inhibitors comparing 6-hour treatment followed by removal of compound (Washout) or 72 hour of prolonged exposure to compound (no washout) (data are presented as means ± s.d. (n=3) biological replicates) using CellTiter Glo f. Quantification of Annexin V-positive MOLT4 cells treated with compounds at 250 nM after 24 hours with and without washout (data represented as means ± s.d. (n=3) biological replicates). g. Immunoblot of proteins shown after 24-hour treatment of MOLT4 cells with 250nM THAL-SNS-032, NVP-2, or SNS-032 with and without washout after 6 hours. Uncut western blots are in Supplementary Figure 7.