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. 2018 Mar 21;7:e32036. doi: 10.7554/eLife.32036

Figure 1. Imiquimod produces behavioral and neuronal responses in the zebrafish.

(A–H) 3dpf live elavl3:CaMPARI TG imaging of larvae exposed to 405 nm light and control (A), 50 μM allyl isothiocyanate (AITC) (B), 100 μM imiquimod (IMQ) (C), 100 μM histamine (HIST) (D), 500 μM serotonin (5-HT) (E), 100 μM SLIGRL-NH2 (F), 100 μM deoxycholic acid (DCA) (G), and 100 μM loxoribine (LOX) solutions (H). (I), Counts of photoconverted cells from experiments (A-H). (J), 5dpf WT larval locomotor behavior screen. (K) Representative traces of adult behavior. (L) Adult lip-rubbing behavioral assay. (I, J, L) ***p<0.001, **p<0.01, one-way ANOVA. Bars represent mean ± s.e.m.

Figure 1.

Figure 1—figure supplement 1. Behavioral effects of noxious pruritic and algogenic stimuli in the zebrafish.

Figure 1—figure supplement 1.

(A) Dose-response curve for IMQ in larval locomotion assay, n = 48 for all conditions. (B) Average velocity of adult zebrafish injected with AITC or known pruritogens. 10 μM AITC induced a significant decrease in swimming velocity, whereas 100 μM IMQ elicited increased velocity. (C) Quantification of freezing behavior in the adult behavioral assay. Of the stimuli tested, only 10 μM AITC elicited significant freezing behaviors in adult zebrafish, although fish injected with 200 μM IMQ displayed a trend towards elevated freezing behaviors. (D) A comparison of lip-rubbing behavior in adult zebrafish injected with 10 μM AITC, 100 μM IMQ, and 100 μM loxoribine. Only IMQ evoked significant lip-rubbing behavior. ***p<0.001, **p<0.01, one-way ANOVA.