Fig. 7.

IRAK-M expression is elevated in asthmatic human samples after Derp1 segmental allergic challenge. a Representative H&E staining and PAS staining of lung biopsies before and after Derp1 segmental challenge, as well as BALF and brush cytospins before and after treatment stained with Giemsa (n = 4). b Cytospin slides of brushing, total BALF cells, BALF CD15 + and CD205 + cells samples were immune stained for IRAK-M. c Quantification of IRAK-M expression in total BALF cells, BALF CD15 + and CD205 + cells samples as in b using Velocity program. d Total BALF cells were immune stained for PIN1 and pS71PIN1 and quantified using Velocity program e. f RNA was extracted from BALF cellular contents and analyzed for the relative expression of Il6, Csf3, Cxcl2 and Ccl5 by qRT-PCR. The data were analyzed by a Student’s two-tailed t test and the values are reported as mean ± standard errors of the means (SEM). *- statistical significance (P < 0.05), **- significance (P < 0.01). Scale bar = 50 µm