Table 4.
Oligonucleotides used in this work.
Name | Sequence 5′ → 3′ |
---|---|
bCp-RASSF1A* | TCGCGCAACCGTGCGAGGTCGGCC–Biotin |
Target RASSF1A | CC(M)GGC(M)GTGGGCC(M)GACCTC(M)GCAC(M)GGTTGC(M)GC(M)GAC(M)GC(M)GTAGC(M)GC |
bCp-MGMT* | Biotin-CACCAAGTCGCAAACGGTGCGCAC |
Target MGMT | GTCCC(M)GAC(M)GCCC(M)GCAGGTCCTC(M)GCGGTGCGCACCGTT |
C(M): 5-methylcytosine (5-mC).
*The positions of biotin in both Cps were selected to place the high number of 5-mCs in the synthetic targets farther away from the surface of MBs to make there more accessible for anti-5-mC recognition.