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. 2018 Apr 23;8:6400. doi: 10.1038/s41598-018-24694-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of epithelialization using fluorescein corneal staining. Two days postsurgery, the iAM surface showed large area of fluorescein staining, whereas on dAM surface the fluorescein uptake was much smaller (2D). Five days after surgery, the central area of iAM surface showed positive staining, while the dAM surface was almost negative (5D). Persistent dots fluorescein staining was observed on iAM even after 2 weeks (2W, arrow), while was completly negative on the dAM (A). Statistical analysis showed the area of epithelialization on dAM vs iAM, there was significant difference in day 2 and day 3 (B, *p < 0.05, **p < 0.01).