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. 2018 Apr 23;8:6400. doi: 10.1038/s41598-018-24694-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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H&E staining on rabbit corneal and conjunctival tissue after AM transplantation. Visible stratified epithelium was observed 2 weeks post surgery with iAM (A) and dAM (B). Uniform epithelial layer and intensified stromal cellularization was observed in iAM group 3 weeks post surgery. In addition, the stroma showed folded dense matrix like embedded structure (C, blue arrow head). Three weeks post surgery sections of dAM transplanted group showed smooth and homogenous stroma (D). Inserted figures show high magnification of the dotted portion (a,b,c,d). The black arrow heads show the corneal limbal junction. Bar represents 500 μm in (A–D), and represents 100 μm in a,b,c, and d.