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. 2018 Apr 23;8:6400. doi: 10.1038/s41598-018-24694-4

Figure 9.

Figure 9

Macrophage infiltration in the AM loci after AM transplantation. Anti-macrophage antibody immunostaining (green fluorescence) showed infiltration of macrophages in the stroma of iAM transplantation loci (A) at 4 days (4D) that increased at 5 days (5D), and were found disseminated the entire stroma at 3 weeks (3 W). However, macrophage infiltration was absent in the dAM transplantation loci (B) 5 days post surgery, a few macrophages can be observed in the stroma 2 weeks post surgery (2W), and a gradual increase at 3 weeks (3W). DAPI staining (blue fluorescence) demonstrated cellularity of the tissues. Bar represents 20 μm. IOD analysis (C) showed significant difference in the rate of macrophage infiltration between the two groups post surgery at each time point (B, *p < 0.05, **p < 0.01, ***p < 0.001).